In vitro cell behavior of osteoblasts on Pyrost bone substitute

Polyurethanes have the potential to impart cell-relevant properties like excellent biocompatibility, high and interconnecting porosity and controlled degradability into biomaterials in a relatively simple way. In this context, a biodegradable composite material made of an isocyanate-terminated co-oligoester prepolymer and precipitated calcium carbonated spherulites (up to 60% w/w) was synthesized and investigated with regard to an application as bone substitute in dental and orthodontic application. After foaming the composite material, a predominantly interconnecting porous structure is obtained, which can be easily machined. The compressive strength of the foamed composites increases with raising calcium carbonate content and decreasing calcium carbonate particle size. When stored in an aqueous medium, there is a decrease in pressure stability of the composite, but this decrease is smaller the higher the proportion of the calcium carbonate component is. In vitro cytocompatibility studies of the foamed composites on MC3T3-E1 pre-osteoblasts revealed an excellent cytocompatibility. The in vitro degradation behaviour of foamed composite is characterised by a continuous loss of mass, which is slower with higher calcium carbonate contents. In a first pre-clinical pilot trial the foamed composite bone substitute material (fcm) was successfully evaluated in a model of vertical augmentation in an established animal model on the calvaria and on the lateral mandible of pigs.

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P44. A new method for the in vitro assessment of bone substitute materials

Bone ◽

10.1016/8756-3282(92)90447-5 ◽

1992 ◽

Vol 13 (1) ◽

pp. 117-118

Author(s):

RL Sammons

Keyword(s):

Bone Substitute ◽

New Method ◽

Bone Substitute Materials ◽

In Vitro Assessment ◽

Substitute Materials

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Comparación de la citocompatibilidad in vitro entre los biomateriales, fibroína y polipropileno

Revista Facultad de Ingeniería ◽

10.19053/01211129.v26.n45.2017.6056 ◽

2017 ◽

Vol 26 (45) ◽

Author(s):

Alejandro Arboleda-Carvajal ◽

Julián González ◽

Manuel Hernando Franco-Arias ◽

Liliana Valladares-Torres

Keyword(s):

Cell Proliferation ◽

Statistical Difference ◽

Indirect Evidence ◽

Live Cells ◽

Cell Behavior ◽

In Vitro Methods ◽

Polypropylene Material ◽

Metabolic Reduction ◽

Number Of Cells

This study evaluates the cell behavior of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture much fibroin material such as polypropylene, as the number of cells / sample was performed by the metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene material, a statistical difference was found in the average number of live cells for fibroin sample regardless of the type of test (p<0.005). By the use of in vitro methods, it is shown that fibroin material has better cell behavior in terms of viability, compared with polypropylene.

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Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats

Nanomaterials ◽

10.3390/nano10050918 ◽

2020 ◽

Vol 10 (5) ◽

pp. 918

Author(s):

Smiljana Paraš ◽

Dijana Trišić ◽

Olivera Mitrović Ajtić ◽

Bogomir Prokić ◽

Damjana Drobne ◽

...

Keyword(s):

Comet Assay ◽

Bone Substitute ◽

Calcium Hydroxyapatite ◽

Volume Density ◽

Future Application ◽

Oral Exposure ◽

Numerical Density ◽

High Porosity ◽

Toxicological Profile

Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.

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Isolation, Characterization, and Agent-Based Modeling of Mesenchymal Stem Cells in a Bio-construct for Myocardial Regeneration Scaffold Design

Data ◽

10.3390/data4020071 ◽

2019 ◽

Vol 4 (2) ◽

pp. 71 ◽

Cited By ~ 1

Author(s):

Diana Victoria Ramírez López ◽

María Isabel Melo Escobar ◽

Carlos A. Peña-Reyes ◽

Álvaro J. Rojas Arciniegas ◽

Paola Andrea Neuta Arciniegas

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

In Silico ◽

Cardiac Tissue ◽

Cellular Level ◽

Cell Behavior ◽

Agent Based Model ◽

Agent Based ◽

And Migration

Regenerative medicine involves methods to control and modify normal tissue repair processes. Polymer and cell constructs are under research to create tissue that replaces the affected area in cardiac tissue after myocardial infarction (MI). The aim of the present study is to evaluate the behavior of differentiated and undifferentiated mesenchymal stem cells (MSCs) in vitro and in silico and to compare the results that both offer when it comes to the design process of biodevices for the treatment of infarcted myocardium in biomodels. To assess in vitro behavior, MSCs are isolated from rat bone marrow and seeded undifferentiated and differentiated in multiple scaffolds of a gelled biomaterial. Subsequently, cell behavior is evaluated by trypan blue and fluorescence microscopy, which showed that the cells presented high viability and low cell migration in the biomaterial. An agent-based model intended to reproduce as closely as possible the behavior of individual MSCs by simulating cellular-level processes was developed, where the in vitro results are used to identify parameters in the agent-based model that is developed, and which simulates cellular-level processes: Apoptosis, differentiation, proliferation, and migration. Thanks to the results obtained, suggestions for good results in the design and fabrication of the proposed scaffolds and how an agent-based model can be helpful for testing hypothesis are presented in the discussion. It is concluded that assessment of cell behavior through the observation of viability, proliferation, migration, inflammation reduction, and spatial composition in vitro and in silico, represents an appropriate strategy for scaffold engineering.

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ATLANTIK Genta, a New Concept of Gentamicin Loaded HAP/TCP Bone Substitute for Prophylactic Action-In Vitro Releasing Mechanisms Study

Bioceramics 20 - Key Engineering Materials ◽

10.4028/0-87849-457-x.1203 ◽

2007 ◽

pp. 1203-1206

Author(s):

Aurélien Bignon ◽

F. Laurent ◽

J. Goldnadel ◽

Jérôme Chevalier ◽

Gilbert Fantozzi ◽

...

Keyword(s):

Bone Substitute

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Experimental-Numerical Design and Evaluation of a Vibration Bioreactor Using Piezoelectric Patches

Sensors ◽

10.3390/s19020436 ◽

2019 ◽

Vol 19 (2) ◽

pp. 436 ◽

Cited By ~ 2

Author(s):

David Valentín ◽

Charline Roehr ◽

Alexandre Presas ◽

Christian Heiss ◽

Eduard Egusquiza ◽

...

Keyword(s):

Numerical Model ◽

Mechanical Vibration ◽

Cell Types ◽

Experimental Results ◽

Cell Behavior ◽

Frequency Range ◽

Piezoelectric Patch ◽

Numerical Design ◽

Vibration Pattern

In this present study, we propose a method for exposing biological cells to mechanical vibration. The motive for our research was to design a bioreactor prototype in which in-depth in vitro studies about the influence of vibration on cells and their metabolism can be performed. The therapy of cancer or antibacterial measures are applications of interest. In addition, questions about the reaction of neurons to vibration are still largely unanswered. In our methodology, we used a piezoelectric patch (PZTp) for inducing mechanical vibration to the structure. To control the vibration amplitude, the structure could be excited at different frequency ranges, including resonance and non-resonance conditions. Experimental results show the vibration amplitudes expected for every frequency range tested, as well as the vibration pattern of those excitations. These are essential parameters to quantify the effect of vibration on cell behavior. Furthermore, a numerical model was validated with the experimental results presenting accurate results for the prediction of those parameters. With the calibrated numerical model, we will study in greater depth the effects of different vibration patterns for the abovementioned cell types.

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Osteogenic Potential Using a Malleable, Biodegradable Composite Added Traditional Chinese Medicine: in vitro and in vivo Evaluations

The American Journal of Chinese Medicine ◽

10.1142/s0192415x06004351 ◽

2006 ◽

Vol 34 (05) ◽

pp. 873-886 ◽

Cited By ~ 6

Author(s):

Chun-Hsu Yao ◽

Bai-Shuan Liu ◽

Chau-Guey Liu ◽

Yueh-Sheng Chen

Keyword(s):

Bone Substitute ◽

Bone Growth ◽

In Vitro Study ◽

Osteogenic Potential ◽

Calvarial Bone ◽

Large Defect ◽

Biodegradable Composite ◽

Calvarial Bone Defect

The purpose of this investigation was to prepare and evaluate the feasibility and biocompatibility of a new composite as a large defect bone substitute. The new GTGG was mainly composed of tricalcium phosphate ceramic particles and glutaraldehyde crosslinked gelatin in which Gui-Lu-Jiao was added (a mixture of Cervi Colla Cornus and Colla Plastri Testudinis). In the in vitro study, rat's calvaria osteoblasts were used to study bone characteristics upon exposure to different concentrations of the Gui-Lu-Jiao solution. In the in vivo study, GTGG composites were implanted into the defects of calvarial bones in mature New Zealand rabbits to test their osteogenerative characteristics. As a result, we found that Gui-Lu-Jiao added to the culture could promote the proliferation of osteoblasts. In addition, GTGG could induce a large amount of new bone growth in the rabbit's calvarial bone defect. Therefore, the GTGG composite might be a potential bone substitute.

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